Moreover, particle size distribution, and average pore size (low-temperature nitrogen adsorption, LTNA) of the new very fine core–shell particles were investigated.Īccording to this study, increased flow rates can be applied on sub-2 μm core–shell columns to accomplish very fast separations without significant loss in efficiency. The results obtained with low molecular weight polar neutral analytes (272 g/mol, 875 g/mol), with a polypeptide (4.1 kDa) and with different sized proteins (18.8 kDa, 38.9 kDa and 66.3 kDa) are presented in this study. UHPLC methods developed with fully porous sub-2 m columns often generate backpressure higher than HPLC system limita- tions. Van Deemter, Knox and kinetic plots are calculated. The efficiency of separation was investigated also for small pharmaceutical and large molecules (proteins). In this study a systematical evaluation of the efficiency and achievable analysis time obtained with 5 cm long narrow bore column packed with sub-2 μm core–shell particles (1.25 μm core diameter and 0.23 μm porous silica layer), and other type very efficient columns is presented. The advantages of sub-2 μm particles and shell particles are combined in the new Kinetex 1.7 μm particles. Ascentis Express C18 column (Supelco) with a particle size of 2.7 m (50 mm × 2.1 mm) was purchased from SigmaAldrich Ltd., Budapest. Compatible with All Analytical HPLC/UHPLC Systems. The new Kinetex coreshell columns packed with 2.6 m shell particles (50 mm × 2.1 mm) and with 1.7 m shell particles (50 mm × 2.1 mm) were obtained from GEN-Lab Ltd, Budapest.
Kinetex core shell upgrade#
This versatile column can easily upgrade existing fully porous 5 m and 3 m methods to shorten run time, increase sensitivity, and even provide greater resolution with much higher efficiency levels. Yielding remarkable chromatographic resolution, higher peak capacities, and greater sensitivity, so labs can get the most out of every HPLC or UHPLC analysis. The Kinetex 2.6 m Polar C18 is a core-shell based particle with a polar modified surface and a C18 alkyl phase. Applying particles with shortened diffusion path to improve the efficiency of separation performs higher efficiency than it is possible with the totally porous particles having the same size. The Kinetex C18 is a well-rounded, all purpose core-shell column that produces increased efficiencies over traditional fully porous columns. 4-Fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) was used as a fluorescence derivatization reagent for amino acids, followed by separation on a core-shell Kinetex C18 column. This phase will demonstrate improved peak shape and increased retention of acidic compounds. A fast HPLC method using a core-shell particle column was developed for the determination of amino acids. Low ligand density and an inactive surface make this column a great hydrogen acceptor. At present sub-2 μm packed columns are very popular to accomplish rapid and efficient separations. Kinetex XB-C18 Core Shell A Unique Core-Shell Selectivity Di-isobutyl side chains differentiate this C18 column.
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